![]() Although the commercialization of this crustacean generated an economic revenue of more than 16 million pesos in 2016, the residues generated by the shrimp industry have a negative impact on the environment ( SIAP, 2016). Shrimp production in Latin America was estimated to be between 500,000 and 600,000 tons in 2016, with Mexico being one of the main suppliers ( FAO, 2016). Molecular identification indicated that the isolated strains belong to the species Stenotrophomonas maltophilia. The B4 strain showed the highest specific activity, which was 6,677.07 U/mg protein. Then, strain-specific activity was evaluated. From the above, ten strains were selected. Secondary selection consisted of semi-quantitative evaluation of chitinolytic activity with a drop dilution assay. Strains were selected on colloidal chitin agar medium primarily based on a hydrolysis halo larger than 2 mm and a growing phase no longer than 6 days. These microorganisms were isolated and characterized based on macro- and microscopic morphological traits. The objective of the present study was to isolate, characterize and identify microorganisms with high chitinolytic activity. Bacteria capable of gradually hydrolyzing chitin into N-acetylglucosamine monomers can have applications in the transformation of residues from shrimp and other crustaceans. PeerJ 7: e6102 Ĭhitin is the second most abundant organic compound in nature and represents a rich carbon and nitrogen source that is primarily transformed by bacterial communities. Isolation and identification of marine strains of Stenotrophomona maltophilia with high chitinolytic activity. Cite this article Salas-Ovilla R, Gálvez-López D, Vázquez-Ovando A, Salvador-Figueroa M, Rosas-Quijano R. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. Licence This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. Instituto de Biociencias, Universidad Autónoma de Chiapas, Tapachula, Chiapas, Mexico DOI 10.7717/peerj.6102 Published Accepted Received Academic Editor Corinne Maurice Subject Areas Microbiology, Molecular Biology Keywords Chitinolytic strains, Seawater, Chitinases, Specific activity, Shrimp residues Copyright © 2019 Salas-Ovilla et al.
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